ROTENONE
C23 H22 O6
MW: 394.43
CAS: 83-79-4
METHOD: 5007, Issue 2
5007
RTECS: DJ2800000
EVALUATION: FULL
OSHA : 5 mg/m 3 NIOSH: 5 mg/m 3; Group II Pesticide ACGIH: 5 mg/m 3
PROPERTIES:
Issue 1: 15 February 1984 Issue 2: 15 August 1994
solid, MP 163 °C or 181 °C; BP 220 °C @ 0.5 mm Hg; d ca. 1 g/cm 3; VP not significant
SYNONYMS: tubatoxin; cube
SAMPLING SAMPLER:
FILTER (1-mm PTFE membrane)
FLOW RATE:
1 to 4 L/min
VOL-MIN: -MAX:
8L 400 L
SHIPMENT:
routine
SAMPLE STABILITY:
MEASUREMENT
at least 7 days @ 25 °C in dark
BLANKS:
2 to 10 field blanks per set
BULK SAMPLE:
desirable; 1 g
ACCURACY 3
RANGE STUDIED:
1 to 11 mg/m [1] (100-L sample)
BIAS:
- 0.6%
ˆ ): OVERALL PRECISION (S rT
0.079
ACCURACY:
± 13.5%
TECHNIQUE:
HPLC; UV DETECTION
ANALYTE:
Rotenone
EXTRACTION:
4 mL acetonitrile; 30 min
INJECTION VOLUME:
10 µL
MOBILE PHASE:
60% methanol/40% water, 2 mL/min
DETECTOR:
UV @ 290 nm; 0.1A full-scale; 1-cm cell
COLUMN:
µ-Bondapak C 18 (30 cm x 3.9-mm ID stainless steel); ambient temperature
CALIBRATION:
solutions of Rotenone in acetonitrile
RANGE:
0.04 to 1 mg per sample
ESTIMATED LOD:
4 µg per sample [1,2]
PRECISION (Sr):
0.024 [1]
APPLICABILITY: The working range is 0.4 to 10 mg/m 3 for a 100-L air sample and the method is applicable to commercial formulations.
INTERFERENCES: None known. Rotenone, a naturally occurring insecticide, is adequately separated by HPLC from other compounds (e.g., sumatrol, α-toxicarol, deguelin, elliptone, malaccol, and tephrosin [3]) present in commercial cube root extracts [4]. Rotenone is sensitive to photodecomposition.
OTHER METHODS: This is Method S300 [2] in a revised format.
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
