PYRETHRUM
C20 H28 O3 to C22 H30 O5
MW: 316.4 to 372.4
(Table 1)
5008
CAS: 8003-34-7
RTECS: UR4200000
(active constituents)
METHOD: 5008, Issue 2
EVALUATION: FULL
OSHA : 5 mg/m 3 NIOSH: 5 mg/m 3; Group II pesticide ACGIH: 5 mg/m 3
PROPERTIES:
Issue 1: 15 May 1985 Issue 2: 15 August 1994
viscous brown resin or solid; VP not significant
SYNONYMS: Active constituents include pyrethrin I and II, jasmolin I and II, and cinerin I and II.
SAMPLING
MEASUREMENT
SAMPLER:
FILTER (glass fiber)
TECHNIQUE:
HPLC, UV DETECTION
FLOW RATE:
1 to 4 L/min
ANALYTE:
six active constituents of pyrethrum
VOL-MIN: -MAX:
20 L 400 L
EXTRACTION:
10 mL acetonitrile; stand 30 min
SHIPMENT:
routine; ship bulk sample separately
INJECTION VOLUME:
25 µL
SAMPLE STABILITY:
FIELD BLANKS:
COLUMN:
C 18 reverse phase, 10-µm packing, 25 to 30 cm
MOBILE PHASE:
85% acetonitrile/15% water, isocratic, 1.0 mL/min, room temperature, 2800 kPa (400 psi)
DETECTOR:
UV absorption @ 225 nm
CALIBRATION:
solutions of pyrethrum in acetonitrile
RANGE:
0.1 to 1.8 mg per sample
at least 1 week @ 25 °C [1]
2 to 10 field blanks per set
ACCURACY 3
RANGE STUDIED:
1.4 to 8.5 mg/m [1] (132-L samples)
BIAS:
- 4.5% ESTIMATED LOD: 0.01 mg per sample
ˆ rT): 0.070 [1] OVERALL PRECISION (S PRECISION (Sr): ACCURACY:
0.040 [1]
± 13.8%
APPLICABILITY: The working range is 0.5 to 10 mg/m 3 for a 200-L air sample.
INTERFERENCES: Specific interferences have not been studied. Mass spectrometry or gas-liquid chromatography with electron-capture detection may be needed for confirmation [1].
OTHER METHODS: This method is S298 [2] in a revised format. For bulk samples, gas-liquid chromatography with electron-capture detection has been recommended [3]. A modified HPLC method (C 8 column, programmed methanol/water or acetonitrile, isocratic) has been used [4].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
