STRYCHNINE
C21 H22 N2O2
MW: 334.42
METHOD: 5016, Issue 2
CAS: 57-24-9
5016
RTECS: WL2275000
EVALUATION: FULL
OSHA : 0.15 mg/m 3 NIOSH: 0.15 mg/m 3/10 h; Group I Pesticide ACGIH: 0.15 mg/m 3
PROPERTIES:
Issue 1: 15 May 1985 Issue 2: 15 August 1994
solid; MP 268 °C; VP not significant
SYNONYMS: strychnidin-10-one
SAMPLING SAMPLER:
FILTER (glass fiber, 37-mm)
FLOW RATE:
1 to 3 L/min
VOL-MIN: -MAX:
70 L @ 0.15 mg/m 3 1000 L
SHIPMENT:
routine
SAMPLE STABILITY:
at least 7 days @ 25 °C [1]
BLANKS:
2 to 10 field blanks per set
ACCURACY RANGE STUDIED:
0.073 to 0.34 mg/m 3 [3] (180-L samples)
BIAS:
4.4%
ˆ ): 0.059 [1] OVERALL PRECISION (S rT ACCURACY:
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
strychnine
DESORPTION:
5 mL mobile phase
INJECTION VOLUME:
20 µL
COLUMN:
25 cm x 4.2-mm ID, packed with µBondpack C18, 10-µm particle size
MOBILE PHASE:
aqueous 1-heptane sulfonic acid + CH 3CN; pH 3.5; 1 mL/min @ ambient temperature
DETECTOR:
UV absorption @ 254 nm
CALIBRATION:
solutions of strychnine in mobile phase
RANGE:
10 to 70 µg per sample
ESTIMATED LOD: 0.8 µg per sample [1,2] PRECISION (Sr):
0.042 @ 13 to 62 µg per sample [1]
± 14.6%
APPLICABILITY: The working range is 0.05 to 10 mg/m 3 for a 200-L air sample.
INTERFERENCES: None known.
OTHER METHODS: This revises Method S302 [2].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
