PENTAMIDINE ISETHIONATE
C23H36N4S2O10
MW: 592.75
METHOD: 5032, Issue 2
CAS: 140-64-7
5032
RTECS: CV6500000
EVALUATION: PARTIAL
OSHA : no PEL NIOSH: no REL ACGIH: no TLV
PROPERTIES:
Issue 1: 15 May 1989 Issue 2: 15 August 1994
solid; MP 192.3 - 193.7 °C; soluble in water and ethanol
SYNONYMS: 4,4'-[1,5-pentanediylbis(oxy)]bisbenzenecarboximidamide bis(2-hydroxyethanesulfonate); Pentam 300
SAMPLING
MEASUREMENT
SAMPLER:
FILTER (37-mm, PVC in opaque cassette)
TECHNIQUE:
HPLC, FLUORESCENCE DETECTION
FLOW RATE:
1 to 2 L/min
ANALYTE:
pentamidine isethionate
VOL-MIN: -MAX:
50 L @ 1 µg/m 3 1500 L
RECOVERY:
3 mL of 50:50 ethanol:water, 0.085% H 3 PO 4 and 0.04% TMAC; ultrasonic bath 10 min
SHIPMENT:
routine INJECTION VOLUME:
SAMPLE STABILITY:
≥ 27 days @ 25 °C in the dark
BLANKS:
2 to 10 field blanks per set
100 µL
MOBILE PHASE:
19.6:80.4 CH 3 CN: water, 0.085% H 3 PO 4 and 0.04% TMAC; 1 mL/min
COLUMN:
Ultrasphere C-8, 5-µm particles, 25 cm x 4.6-mm; ultrasphere C-8 guard
DETECTOR:
fluorescence excitation/emission: 270/340 nm
ACCURACY RANGE STUDIED:
none
CALIBRATION:
pentamidine isethionate in eluent
BIAS:
not determined
RANGE:
50 to 3,000 ng per sample
ˆ ): not determined OVERALL PRECISION (S rT
ESTIMATED LOD: 18 ng per sample
ACCURACY:
PRECISION (Sr):
not determined
0.065
APPLICABILITY: The working range is 0.5 to 30 µg/m 3 for a 100-L air sample. This method has been used to measure pentamidine isethionate in air in a hospital [1].
INTERFERENCES: None identified.
OTHER METHODS: None published for air samples. The method for measurement in solution is a variation of the method of Lin et al. [2].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94
