POLYNUCLEAR AROMATIC HYDROCARBONS by HPLC
Formulae: Table 1
MW: Table 1
METHOD: 5506, Issue 3
RTECS: Table 2
EVALUATION: PARTIAL
OSHA : Table 3 NIOSH: Table 3 ACGIH: Table 3 Compounds
CAS: Table 2
PROPERTIES:
acenaphthene acenaphthylene anthracene benz[a]anthracene benzo[b]fluoranthene benzo[k]fluoranthene
5506
Issue 1: 15 May 1985 Issue 3: 15 January 1998 Table 1
benzo[ghi]perylene benzo[a]pyrene benzo[e]pyrene chrysene dibenz[a,h]anthracene fluoranthene
fluorene indeno[1,2,3-cd]pyrene naphthalene phenanthrene pyrene
NAMES & SYNONYMS: Polycyclic aromatic hydrocarbons, PAHs; also see Table 2.
SAMPLING SAMPLER: FILTER + SORBENT TUBE (37-mm, 2-µm, PTFE + washed XAD-2, 100 mg/50 mg) FLOW RATE:
2 L/min
VOL-MIN: -MAX:
200 L 1000 L
SHIPMENT:
transfer filters to culture tubes; wrap sorbent and culture tubes in Al foil; ship @ 0 C
MEASUREMENT TECHNIQUE:
HPLC, FLUORESCENCE/UV DETECTION
ANALYTE:
compounds listed above
EXTRACTION:
5 mL acetonitrile; ultrasonic bath, 30 to 60 minutes
INJECTION VOLUME:
SAMPLE STABILITY:
unknown; protect from heat and UV light
FIELD BLANKS: MEDIA BLANKS:
3 to 10 field blanks per set 6 to 10 media blanks per set
10 to 50 µL
MOBILE PHASE:
acetonitrile/water gradient @ ambient temperature, 1 mL/min
COLUMN:
250 x 4.6-mm, reversed-phase, 5-µm C18
DETECTOR:
UV @ 254 nm; fluorescence @ 340 nm (excitation), 425 nm (emission)
CALIBRATION:
standards in acetonitrile
RANGE:
see EVALUATION OF METHOD
ACCURACY RANGE STUDIED: not determined BIAS: OVERALL PRECISION ( ACCURACY:
not determined
rT
):
not determined
ESTIMATED LOD: see EVALUATION OF METHOD PRECISION ( r):
see EVALUATION OF METHOD
not determined
APPLICABILITY: This method is applicable to samples that can be extracted with acetonitrile. This method is not applicable to samples that require a different extraction solvent or contain large amounts of highly adsorptive particulate matter, e.g., fly ash or diesel soot; also, this method is not applicable to asphalt fume samples.
INTERFERENCES: Any compound that elutes at the same HPLC retention time may interfere. Heat, ozone, NO2, or UV light may cause sample degradation.
OTHER METHODS: This revises P&CAM 206 and 251 [1]. Method 5515 uses the same sampling technique, with gas chromatographic measurement [2]. Method 5800 uses the same sampling technique, and a flow-injection method to determine total polycyclic aromatic compounds at two different sets of fluorescent wavelengths [3].
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition
