3-BROMOPROPIONIC ACID in URINE: METHOD 8324, Issue 1, dated 29 August 2014 - Page 5 of 9
longer time frame of two months or more. Derivatized sample extracts appeared to be stable during a one week time frame. Range: This method should be considered accurate for the estimation of 3-BPA in human urine within the 2.0 to 100 μg/mL method validation range. REFERENCES: [1] ACGIH [2013]. TLVs and BEIs based on the documentation of the Threshold Limit Values for chemical substances and physical agents and Biological Exposure Indices. Cincinnati, Ohio: American Conference of Governmental Industrial Hygienists. www.acgih.org [Date accessed: April 2014.] [2] Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area [2013]. List of MAK and BAT values 2013; Maximum Concentrations and Biological Tolerance Values at the Workplace. DFG: Deutsche Forschungsgemeinschaft, Wiley-VCH Verlag GmbH & Co. http:// www.dfg.de/en/dfg_profile/publications/senate_commissions/index.html#micro7535782 [Date accessed April 2014.] [3] SUVA (Swiss National Accident Insurance Fund) [2013]. Protection de la santé au poste de travail. Valeurs limtes d’exposition aux postes de travail. http://www.suva.ch/english/startseite-en-suva/ praevention-en-suva/publications-en-suva.htm [Date accessed: April 2014.] [4] Institut für Arbeitsschutz der Deutschen Gesetzlichen Unfallversicherung (IFA) http://www.dguv. de/ifa/Gefahrstoffdatenbanken/GESTIS-Internationale-Grenzwerte-für-chemische-Substanzenlimit-values-for-chemical-agents/index-2.jsp [Date accessed: April 2014.] [5] US FDA [2001]. Guidance for industry: Bioanalytical method validation. U.S. Food and Drug Administration, Washington, DC. http://www.fda.gov/downloads/Drugs/Guidances/ucm070107. pdf [Date accessed April 2014]. [6] B’Hymer C [2004]. 3-Bromopropionic acid in urine, Backup data report, Method 8324. Internal report. [7] Jones AR, Walsh DA [1979]. The oxidative metabolism of 1-bromopropane in the rat. Xenobiotica 9(12): 763-772. [8] Mathias PI, Cheever KL, Hanley KW, Marlow KL, Johnson BC, B’Hymer C [2012]. Comparison and evaluation of urinary biomarkers for occupational exposure to spray adhesives containing 1-bromopropane. Toxicol Mech Methods 22(7): 526-532. [9] B’Hymer C, Cheever KL [2004]. Development of a gas chromatographic test for the quantification of the biomarker 3-bromopropionic acid in human urine. J Chromatogr B 802: 361-366. [10] CDC [2007]. 2007 Guidelines for isolation precautions: preventing transmission of infectious agents in healthcare settings. http://www.cdc.gov/hicpac/2007IP/2007isolationprecautions.html [Date accessed: April 2013.] METHOD WRITTEN BY: Clayton B’Hymer, PhD, NIOSH/DART/BHAB. Final editing performed by Dale A. Shoemaker, PhD, NIOSH/ DART/CEMB. Disclaimer: Mention of any company or product does not constitute endorsement by the National Institute for Occupational Safety and Health. In addition, citations to Web sites external to NIOSH do not constitute NIOSH endorsement of the sponsoring organizations or their programs or products. Furthermore, NIOSH is not responsible for the content of these Web sites. All Web addresses referenced in this document were accessible at the time of publication.
- Note: Because exposure limits and guidelines may change over time, NIOSH recommends referring to the following sources
for updated limits and guidelines on the use of this compound as a marker for 1-bromopropane [1,2,3,4].
NIOSH Manual of Analytical Methods (NMAM), Fifth Edition
