Supposing an infusion intrinsically barren, but readily susceptible of putrefaction when exposed to common air, to be brought into contact with this unilluminable air, what would be the result? It would never putrefy. It might, however, be urged that the air is spoiled by its violent calcination. Oxygen passed through a spirit-lamp flame is, it may be thought, no longer the oxygen suitable for the development and maintenance of life. We have an easy escape from this difficulty, which is based, however, upon the unproved assumption that the air has been affected by the flame. Let a condensed beam be sent through a large flask or bolt-head containing common air. The track of the beam is seen within the flask—the dust revealing the light, and the light revealing the dust. Cork the flask, stuff its neck with cotton-wool, or simply turn it mouth downward and leave it undisturbed for a day or two. Examined afterward with the luminous beam, no track is visible; the light passes through the flask as through a vacuum. The floating matter has abolished itself, being now attached to the interior surface of the flask. Were it our object, as it will be subsequently, to effectually detain the dirt, we might coat that surface with some sticky substance. Here, then, without "torturing" the air in any way, we have found a means of ridding it, or rather of enabling it to rid itself, of floating matter.
We have now to devise a means of testing the action of such spontaneously purified air upon putrescible infusions. Wooden chambers, or cases, are accordingly constructed having glass fronts, side-windows, and back-doors. Through the bottoms of the chambers test-tubes pass air-tight, their open ends, for about one-fifth of the length of the tubes, being within the chambers. Provision is made for a free connection through sinuous channels between the inner and the outer air. Through such channels, though open, no dust will reach the chamber. The top of each chamber is perforated by a circular hole two inches in diameter and closed air-tight by a sheet of India-rubber. This is pierced in the middle by a pin, and through the pin-hole is pushed the shank of a long pipette, ending above in a small funnel. The shank also passes through a stuffing-box of cotton-wool moistened with glycerine; so that, tightly clasped by the rubber and wool, the pipette is not likely in its motions up and down to carry any dust into the chamber. The annexed woodcut shows a chamber with six test-tubes, its side-windows to w w, its pipette p C, and its sinuous channels a b which connect the air of the chamber with the outer air.
The chamber is carefully closed and permitted to remain quiet for two or three days. Examined at the beginning by a beam sent through its windows, the air is found laden with floating matter, which in three days has wholly disappeared. To prevent its ever rising again into the chambers, the internal surface is coated with glycerine. The fresh but putrescible liquid is introduced into the six tubes in succession by means of the pipette. Permitted to remain