the microbe-ferment of the butyric fermentation was also the agent in decomposition? He would prepare an artificial liquid, consisting of phosphate of potash, magnesia, and sulphate of ammonia, added to the solution of fermentable matter, and in the medium thus formed would develop the microbe-ferment from a pure sowing of it. The microbe would multiply and provoke fermentation. From this liquid he would pass to a second and then to a third fermentable solution of the same composition, and so on, and would find the butyric fermentation appearing in each successively. This method had been sovereign in his studies since 1857. He now proposed to isolate the microbe of blood infected with carbuncle, cultivate it in a pure state, and study its action on animals." As he was still suffering from a partial paralysis, he employed M. Joubert to assist him and share his honors. In April, 1877, he claimed before the Academy of Sciences that he had demonstrated, beyond the possibility of a reply, that the bacillus discovered by Davaine and Rayer in 1850 was in fact the only agent in producing the disease. It still remained to reconcile the facts adduced by Messrs. Jaillard and Leplat with this assertion. The animals which they had inoculated died, but no bacteria could be found in them. M. Paul Bert, in similar experiments, had found a disease to persist after all bacteria had been destroyed. An explanation of the discrepancy was soon found.
The bacteria of carbuncle are destroyed as soon as putrefaction sets in. The virus with which these gentlemen had experimented was taken from animals that had been dead twenty-four hours and had begun to putrefy. They had inoculated with putrefaction, and produced septicæmia instead of carbuncle. All the steps in this line of argument were established by irrefragable proof. M. Pasteur afterward had a similar controversy with some physicians of Turin, at the end of which they shrank from the test experiment he offered to go and make before them. "Remember," shortly afterward said a member of the Academy of Sciences to a member of the Academy of Medicine, who was going—in a scientific sense—to "choke" M. Pasteur, "M. Pasteur is never mistaken."
Having discovered and cultivated the microbe that produces hen-cholera, Pasteur turned his attention to the inquiry whether it would be possible to apply a vaccination to the prevention of these terrible diseases of domestic animals. He found that he could transplant the microbe of hen-cholera to an artificially prepared medium and cultivate it there, and transplant it and cultivate it again and again, to the hundredth or even the thousandth time, and it would retain its full strength—provided too long an interval was not allowed to elapse between the successive transplantations and cultures. But if several days or weeks or months passed without a renewal of the medium, the culture being all the time exposed to the action of oxygen, the infection gradually lost in intensity. A virus was produced of a strength
