tional significance in development, owing to the principle of relation of functional area to mass. It has also another very important function as an isolating factor. The localizations that arise, owing to the organization process of which we have spoken, are rendered relatively stable and permanent by the formation of cell-walls. Thus the elements of the mosaic are isolated, and each isolated part has the opportunity to grow into a new mosaic. Cell-division is thus an important factor in progressive differentiation, not as a cause, but as a means.
4. Environment.—Environment must be conceived in a somewhat broader sense than usual in considering the individual development. The developing embryo has an environment in the usual sense, consisting of all those external conditions that surround it, some of which enter into its development. But in addition to this extra-organic environment there is an intra-organic one; the developing embryo is not merely a unit on which an extra-organic environment operates, but it is a living mosaic, each element of which may conceivably enter into the development of any other in the sense of being a factor in the process. Each part of the embryo, therefore, has an intra-organic environment consisting of all the other parts, 6ome of which constitute relatively immediate environmental factors, others relatively remote ones.
To illustrate: nerves arise in the embryo from certain centers and grow out in the embryonic tissues, much as roots grow out in the soil; the muscles arise separately and the nerves grow to them and make the proper connections. Is this due to an innate tendency of each nerve to grow in particular paths and branch according to definite laws, or, on the other hand, is it due to a directive stimulus exerted on the growing nerve by developing muscle tissue? The answer can be given only by a suitable experiment: If an abnormal innervation area were brought into the field of growth of a developing nerve, would the nerve entering the abnormal area follow its normal mode of branching, or the one characteristic of the normal nerves of the transposed area? To be specific: the bud of a leg of a tadpole that has as yet no nerves may be transplanted to any region of the body (Braus and Harrison), and it develops as a leg; but it receives its innervation from the nerves of the region to which it has been transplanted, and the mode of branching of the nerve is that of the leg nerves. We may generalize this statement by saying that any nerve may be made to depart from its normal mode of branching and to branch like leg nerves, by bringing a leg bud into its innervation area at the time that the nerve is still growing.
It will be seen that if this is generally true, the constancy of distribution of peripheral nerves is not due to the transmission of nerve-branching determinants from generation to generation, but is a function of the intra-organic environment in each generation.
