of putrefaction, (and a fortiori incapable of the chemical actions which constitute life), and the consequent is death, either of the whole organism, or of some portion of it; let us compare with these cases other cases, as much resembling them as possible, but in which that effect is not produced. And, first, "many insoluble basic salts of arsenious acid are known not to be poisonous. The substance called alkargen, discovered by Bunsen, which contains a very large quantity of arsenic, and approaches very closely in composition to the organic arsenious compounds found in the body, has not the slightest injurious action upon the organism." Now when these substances are brought into contact with the tissues in any way, they do not combine with them; they do not arrest their progress to decomposition. As far, therefore, as these instances go, it appears that when the effect is absent, it is by reason of the absence of that antecedent which we had already good ground for considering as the proximate cause.
But the rigorous conditions of the Method of Difference are not yet satisfied; for we can not be sure that these unpoisonous bodies agree with the poisonous substances in every property, except the particular one of entering into a difficultly decomposable compound with the animal tissues. To render the method strictly applicable, we need an instance, not of a different substance, but of one of the very same substances, in circumstances which would prevent it from forming, with the tissues, the sort of compound in question; and then, if death does not follow, our case is made out. Now such instances are afforded by the antidotes to these poisons. For example, in case of poisoning by arsenious acid, if hydrated peroxide of iron is administered, the destructive agency is instantly checked. Now this peroxide is known to combine with the acid, and form a compound, which, being insoluble, can not act at all on animal tissues. So, again, sugar is a well-known antidote to poisoning by salts of copper; and sugar reduces those salts either into metallic copper, or into the red sub-oxide, neither of which enters into combination with animal matter. The disease called painter's colic, so common in manufactories of white-lead, is unknown where the workmen are accustomed to take, as a preservative, sulphuric acid lemonade (a solution of sugar rendered acid by sulphuric acid). Now diluted sulphuric acid has the property of decomposing all compounds of lead with organic matter, or of preventing them from being formed.
There is another class of instances, of the nature required by the Method of Difference, which seem at first sight to conflict with the theory. Soluble salts of silver, such for instance as the nitrate, have the same stiffening antiseptic effect on decomposing animal substances as corrosive sublimate and the most deadly metallic poisons; and when applied to the external parts of the body, the nitrate is a powerful caustic, depriving those parts of all active vitality, and causing them to be thrown off by the neighboring living structures, in the form of an eschar. The nitrate and the other salts of silver ought, then, it would seem, if the theory be correct, to be poisonous; yet they may be administered internally with perfect impunity. From this apparent exception arises the strongest confirmation which the theory has yet received. Nitrate of silver, in spite of its chemical properties, does not poison when introduced into the stomach; but in the stomach, as in all animal liquids, there is common salt; and in the stomach there is also free muriatic acid. These substances operate as natural antidotes, combining with the nitrate, and if its quantity is not too great, immediately converting it into chloride of silver, a substance very slightly
