32. Curved rods; motile by means of polar flagella 33 Straight rods 34 33. Oxidize hydrogen sulfide, depositing sulfur inside the cell Thiospira -p. 82 Note: If Thiospira should prove to be anaerobic, this insertion would be in- valid. Do not deposit sulfur inside the cell Vibrio p. 229 Note: Spencer states that species of Photobacterium are also curved. 34. Acid produced from lactose within 40 days 35 No acid produced from lactose 71 35. Agar is digested 36 Agar is not digested; organisms parasitic on the cytoplasm of the rhizopod Pelomyxa palustris Bacterium* parapelomyxae p. 929 Not as above 38 36. Yellow colonies 37 37. Polar flagella Pseudomonas p. 89 (Including P. segnis and P. lacunogenes, placed in the appendix to Xanthomonas) Motile by means of peritrichous flagella or non-motile. . . . Agarbacteriuni p. 322 38. Motile at 37° or at 20°C 39 Non-motile 59 Motility not recorded (See Xanthomonas clerodendri and X. sesbaniae.) 39. Acid and gas from glucose 40 Acid only from glucose 52 40. Gelatin liquefied 41 Gelatin not liquefied 46 41. Flagella polar; colorless colonies; litmus milk acid, coagulated and slowly digested; 2-3-butj'lene-glycol produced from glucose; cause a fatal septicemia in fish and frogs Aeromonas p. 189 Flagella polar; colorless colonies; not as above Pseudomonas p. 89 Flagella polar; yellow colonies possibly Xanthomonas p. 152 (X. hemmiana) Flagella peritrichous; colonies colorless 42 42. Protopectinase produced Erwinia p. 349 Protopectinase is not produced 43 43. Lactose fermented in 2 days 44 Lactose fermentation delayed Paracolobactrum p. 346 {P. arizonae and P. aerogenoides strains 4611, 1721 and 19111; see also Erwinia chrysanthemi and Arizona Group, Rome, 1953.) 44. Methyl-red positive; Voges-Proskauer positive or negative 45 Methyl-red negative; Voges-Proskauer positive Aerobacter p. 341 45. No gas produced from lactose within 2 days Paracolobactrum p. 346 (P. intermedium strains 8011, 13311, 11411) Gas produced from lactose within 2 days Escherichia p. 335 (E . freundii) (See also Arizona Group, Rome, 1953.) Note: Erwinia carnegieana and Erwinia atroseptica may terminate here. 46. Flagella polar; litmus milk unchanged; starch hydrolyzed; hydrogen sulfide pro- duced; wide range of sugars fermented; organisms will grow in a nitrogen-free medium fixing atmospheric nitrogen Azotomonas p. 198
- Not recognized in the Manual.
