Flagella polar; acid only in lactose; indole-negative; luminescent on a variety of media at pH 8.0 Photobaclerium p. 193 Note: The polar flagellated plant pathogens Fseudomonas petasitis (colorless) and Xanthomonas conjac (yellow pigmented), if it is lactose-positive, may ter- minate here. Not as above: flagella peritrichous 47 47. Alginate fermented with the production of acid and gas; hydrogen sulfide produced; methyl-red positive; Voges-Proskauer weak; citrate utilized; nitrite produced from nitrate Alginobacter p. 348 Not as above 48 48. Methyl-red positive; Voges-Proskauer negative 49 Methyl-red negative; Voges-Proskauer positive 51 Note: Erwinia nimipressuralis and E. dissolvens should be compared with genera terminating between numbers 49 to 51 inclusive. 49. Lactose fermented within 2 days 50 Lactose fermentation delayed Paracolobactruin p. 3^6 P. coZf/orme strains 28221, 551 1,661 1,261 1,31611, 111, 15411, 1811,33811 and 4361. P. intermedium strains 14011, 12611 and 1421. Bethesda and Ballerup Groups, Rome, 1953, for gas-producing strains with slow lactose fermentation. 50. Acid and gas from lactose Escherichia p. 335 {Escherichia, Rome, 1953, for motile strains) Acid only from lactose Paracolobactruin p. 346 (P. intermedium strains 14011 and 12611) 51. Lactose fermented in 2 days Aerobacter p. 341 Lactose fermentation delayed Paracolobactruin p. 346 (P. aerogenoides strains 721, 37711 and 37211) 52. Organisms cause a glanders-like infection (melioidosis) in rats, guinea pigs, rabbits and man; colonies on agar small, circular, slightly raised, thick, opaque and cream- colored with an irregular margin; litmus milk slowly coagulated; blood serum slowly liquefied; optimum temperature, 37°C Pseudomonas p. 89 (P. pseudomallei) Organisms which produce granular conjunctivitis in monkeys and apes and which are believed to be a cause of trachoma in man; optimum temperature, between 28° and 30° C. with motility only at low temperatures; colonies on blood agar small, circular, grayish and translucent, becoming sticky and mucoid Noguchia p. 4^1 Not as above 53 53. Organisms grow in 0.5 per cent peptone water containing a filter paper strip, weaken- ing the latter sufficiently to cause disintegration on slight agitation or reducing it to a pulp; produce clearings around colonies on precipitated cellulose agar plates; cells commonly arranged in angular fashion like corynebacteria Cellulonionas p. 601 (See also Pseudomonas arguta, P. tralucida and also P. ephemerocyanea if the latter produces no pigment.) Not as above 54 54. Colonies yellow 55 Colonies not yellow 57 55. Flagella polar Xanthomonas p. 152 (See also Pseudomonas perlurida, P. suhcreta, P. cepacia and also P. radiciperda and Bacterium tardicrescens should the latter two be lactose fermenters.) Flagella peritrichous 56
