Microaerophilic, facultatively anaerobic. Grows best in the presence of CO2 . Optimum temperature, 37° C. Killed in 10 minutes at 60° C. Non-pathogenic for guinea pigs and mice. Source : Isolated from the blood and heart valve of a case of endocarditis. Habitat: Found in the blood and on the heart valve of one case of endocarditis. 9. Haemophilus influenzae -murium (Kairies and Schwartzer, 1936) Lwoff, 1939. {Bacterium influenzae murium (sic) Kairies and Schwartzer, Cent. f. Bakt., I Abt., Orig., 1S7, 1936, 351; Haemophilus influenzae murium (sic) Lwoff, Ann. Inst. Past., 62, 1939, 168.) in.flu.en'zae-mu'ri.um. Italian noun in- fluenza influenza; L. noun mus, muris mouse; M.L. gen. noun influenzae -murium influenza of mice. Small, short, thick coccobacilli occurring singly and at times in the form of threads. Filterable forms pass through 0.4- to 0.6- micron pores. Non-motile. Shows bipolar staining. Gram-negative. Requires the factor X for growth (Ivano- vics and Ivanovics, Cent. f. Bakt., I Abt., Orig., 139, 1937, 184). Gelatin: No liquefaction. Levinthal agar colonies: Up to 4 mm in diameter, round, convex, glistening, bluish, transparent. Resemble Haemophilus in- fluenzae colonies. Become opaque and whit- ish with age. Blood agar colonies. Whitish. No hemoly- sis. Chocolate agar: Good growth. Endo agar: Red colonies in 2 to 3 days. Levinthal or egg broth: Uniformly turbid. Milk not coagulated. Indole not produced. Acid from glucose, fructose, lactose, maltose and sucrose. No acid from man- nite. Aerobic. Optimum temperature, 37° C. Slightly pathogenic for mice but not for guinea pigs. Serologically homogeneous and distinct from Haemophilus influenzae, Pasteurella and Salmonella typhosa. Source: Isolated from the noses and pharynges of mice. Habitat: Causes conjunctivitis and re- spiratory infections in mice. 10. Haemophilus ovis Mitchell, 1925. (Jour. Amer. Vet. Assoc, 68, 1925, 8.) o'vis. L. fem.n. ovis a sheep; L. gen. noun ovis of a sheep. Small, short, somewhat pleomorphic rods occurring singly and at times in short chains. Coccoid forms occur in old cultures. Non-motile. Gram-negative. Requires the X factor when newly iso- lated. After serial passage on chocolate agar, slight growth on plain agar medium. Primary isolation best on chocholate blood agar. Gelatin slant: Very slight growth in 4 days. Gelatin stab: No growth. Chocolate blood agar colonies: After 24 to 36 hours, pinhead in size, discrete, moist, viscid, translucent; become gray, scaly and adherent to the medium with age. Blood broth: No hemolysis. Plain broth: After adaptation, turbid. Floating ropy strands. Slimy sediment. Litmus milk: No change. Potato: No growth. Indole not produced. Acid but no gas from glucose, fructose, galactose, sucrose, raffinose, sorbitol, man- nitol, mannose and maltose. Weak acidity from lactose and xylose. No acid from arabinose, rhammose, salicin or inositol. Nitrites produced from nitrates. Aerobic. Optimum temperature, 37° C. Slight growth at 28° C. Pathogenic for guinea pigs and sheep. Source: Isolated from the lungs of sheep. Habitat: Causes bronchial pneumonia and generalized hemorrhagic involvement in sheep. 11. Haemophilus putoriorum Haudu- roy et al., 1937. {Bacterium influenzae putoriorum multiforme Kairies, Ztschr. f. Hyg., 117, 1935, 12; Hauduroy et al.. Diet, d. Bact. Path., 1937, 258.) pu.to.ri.o'rum. M.L. mas.n. Putorius
