Page:Bergey's manual of determinative bacteriology.djvu/434

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412
ORDER IV. EUBACTERIALES

generic name of ferret; M.L. gen. pi. noun putoriorvm of ferrets. Coccoid to bacillary rods with a strong pleomorphic tendency. Non-motile. Show liipolar staining. Gram-negative. Growth requirement for V and X un- known. Growth reliable only on media con- taining blood. On ordinary media growth occurs only in the vicinity of other bacteria. Less hemoglobinophilic than is Haemophilus influenzae. Levinthal agar colonies: Young colonies are small with elevated centers and trans- parent peripheries; older colonies are flat with bluish, glassy appearance in trans- mitted light and bluish gray with whitish luster in reflected light. Agar slant: Poor, thin growth when sub- cultured from Levinthal agar. Broth: Lightly turbid; fine granules. Odor like that of Haemophilus influenzae. Non-pathogenic for laboratory animals when inoculated in pure culture. Intra- cutaneous inoculation produces a marked hemorrhagic lesion. Serologically, different strains are re- lated but not homogeneous. Distinct from Haemophilus influenzae and Haemophilus suis. Source: Isolated from the respiratory tracts of ferrets. Habitat: Found in the respiratory tracts of ferrets. 12. Haemophilus ducreyi (Neveu-Le- maire, 1921) Bergey et al., 1923. (Ducrey, Riforma Med., 5, 1889, 98; also see Cong, internat. de dermatol. et syph., Compt. rend., Paris, 1890, 229; Monatsh. f. prakt. Dermatol., 9, 1889, 387; and ibid, 21, 1895, 57, abstract in Cent. f. Bakt., I Abt., 18, 1895, 290; Streptobacillus of soft chancre, Unna, Monatsh. f. prakt. Dermatol., H, 1892, 485; also see ibid, 21, 1895, Ql; Bacillus idceris cancrosi Kruse, in Fliigge, Die Mikroorganismen, 3 Aufi., 2, 1896, 456; Coccobacillus ducreyi Neveu-Lemaire, Precis Parasitol. Hum., 5th ed., 1921, 20; Bergey et al.. Manual, 1st ed., 1923, 271.) du.crey'i. M.L. gen. noun ducreyi of Ducrey; named for A. Ducrey, the bac- teriologist who first isolated this organism. Small rods, 0.5 by 1.5 to 2.0 microns, with rounded ends, occurring singly and in short chains. Non-motile. Gram-negative. Requires the X factor (Lwoff, Ann. Inst. Past., 62, 1939, 168) and other enrichment for growth. Gelatin colonies: No growth. Gelatin stab: No growth. Blood agar colonies: Small, grayish, glis- tening. Slight zone of hemolysis around the colony in three or four days (Teague and Deibert, Jour. Med. Research, 43, 1922, 61). Best growth is obtained on clotted rabbit, sheep or human blood heated to 55° C. for 15 minutes and in casein digest agar con- taining blood (Teague and Deibert, Jour. Urology, 4, 1920, 543). Aerobic, facultatively anaerobic. Optimum temperature, 37° C. Habitat: The cause of soft chancre (chancroid) . 13. Haemophilus haemoglobinophilus (Lehmann and Neumann, 1907) Murray, 1939. (Bacillus haemoglobinophilus canis Friedberger, Cent. f. Bakt., I Abt., Orig., 33, 1903, 401; Bacterium haemoglobinophilus Lehmann and Neumann, Bakt. Diag., 4 Aufl., 2, 1907, 270; Murray, in Manual, 5th ed., 1939, 309.) hae. mo. glo.bi.no 'phi. lus. Gr. noun haema blood; L. noun globus a sphere; M.L. noun haemoglobinum hemoglobin; Gr. adj. philus loving; M.L. adj. haemoglobinophilus hemoglobin-loving. Small rods, 0.2 to 0.3 by 0.5 to 2.0 microns, occurring singly, in pairs and in short chains. Non-motile. Gram-negative. Requires the X factor for growth. Blood agar colonies: Small, clear, trans- parent, entire. Old colonies become opaque. Blood broth: Turbid. Blood milk mixture: Doubtful develop- ment. Indole is produced. Acid but no gas from glucose, fructose, galactose, mannitol, sucrose and xylose. No acid from maltose, lactose, dextrin, arabinose or glycerol (Rivers, Jour. Bact., 7, 1922, 579). Nitrites produced from nitrates. Aerobic, facultatively anaerobic. Optimum temperature, 37° C.