Neumann, Bakt. Diag., 2 Aufl., 2, 1899 (July), 193; Bacterium conjuncHvitidis Chester, Man. Determ. Bact., 1901, 120; not Bacterium conjunctivitidis Migula, Syst. d. Bact., 2, 1900, 509; Diplobacillvs moraxaxenfeld McNab, Klinische Monatsbl. f. Augenheilk., J^2, 1904, 64; Bacillus duplex Hewlett, Med. Res. Council Syst. of Bact., 2, 1929, 417; Lwoff, Ann. Inst. Past., 62, 1939, 173.) la.cu.na'ta. L. noun lacuna a hollow or cavity; M.L. adj. lacunatus pitted. Short rods, 0.4 to 0.5 by 2.0 microns, occurring singly, in pairs and in short chains, with ends rounded or square in the chains. Non-motile. Gram-negative. Requires the addition of blood serum for growth in bouillon or peptone water. Gelatin colonies: No growth. Gelatin stab: No growth. Blood agar colonies: No growth on pri- mar}^ isolation. Growth on subculture is difficult. Certain strains are not surrounded by zones of hemolysis, while others are (Oag, Jour. Path, and Bact., 54, 1942, 128). Serum agar colonies: No growth on pri- mary isolation. Loffler's blood serum: Slow but definite liquefaction with deep pitting around the colonies. Ascitic broth: Turbid with slight, grayish sediment. Blood milk mixture: Doubtful develop- ment. Litmus milk: No growth. Potato: No growth. Various carbohydrates and mannitol are attacked. Aerobic, facultatively anaerobic. Optimum temperature, 37° C. Comments: Audureau (Ann. Inst. Past., 64, 1940, 128) reports a variety of this organ- ism which differs from the parent strain in that it does not liquefy serum. Source: Isolated from conjunctiva. Habitat: Causes subacute infectious con- junctivitis, or angular conjunctivitis. 2. Moraxella liquefaciens (McNab, 1904) Murray, 1948. (Diplobacille liqu^fiant, Pettit, Annales d'oculistique, March, 1899, 166; also see Thesis, Paris, 1900, 223; Diplo- hacilbis liquefaciens McNab, Klinische Monatsbl. f. Augenheilk., 42, 1904, 64; Murray, in Manual, 6th ed., 1948, 591.) li.que.fa'ci.ens. L. part. adj. liquefaciens dissolving. Rods, 1.0 to 1.5 by 2.0 microns, with rounded ends, occurring in pairs and some- times singly. Capsules not demonstrated. Non-motile. Stain uniformly with basic aniline dyes. Gram-negative. Will barely grow in bouillon or peptone water without the addition of blood serum. Gelatin colonies: Round, 1.5 to 2.0 mm in diameter, j-ellowish white. Gelatin stab: Rapid liquefaction. Blood agar: Ready growth in primarj- and subculture. Ascitic agar colonies: Grayish, thick, round, viscous. Peptone agar colonies: Same as above, but less abundant growth. Coagulated serum: Liquefaction in 3 to 4 days; eventually complete. Plain broth: Poor growth, if any. Slight, uniform turbidity. Ascitic broth: Abundant growth in 24 hours at 35° C. Uniform turbidity. Later sediment and an opaque pellicle. Milk: No growth. No coagulation. Potato: Slight, yellowish white, viscous growth. Optimum temperature, between 20° and 37° C. Killed at 55° C for 15 minutes. Aerobic. Not pathogenic for laboratory animals. Source: Isolated from cases of conjuncti- vitis associated with corneal ulceration in man. Habitat: Conjunctivitis in man so far as known. 3. Moraxella bovis (Hauduroy et al., 1937) Murray, 1948. (Diplobacillus, Allen, Jour. Amer. Vet. Med. Assn., 54, 1918, 307; Diplobacillus, Jones and Little, Jour. Exp. Med., 38, 1923, 139; Hemophilus bovis Hau- duroy et al.. Diet. d. Bact. Path., 1937, 247; Murray, in Manual, 6th ed., 1948, 591.) bo'vis. L. noun bos cow, ox; L. gen. noun bovis of an ox or cow. Short, plump rods, 0.5 by 1.5 to 2.0 mi- crons, with rounded ends, usually occurring in pairs and in short chains. Encapsulated. Non-motile. Gram-negative.
