Gelatin: Slow growth at 22° C. Ver}^ slow liquefaction. Blood agar colonies: After 24 hours, round, translucent, grayish white, sur- rounded by a narrow, clear zone of hemoly- sis ; deep colonies tiny with a clear hemolytic zone, usually 1.5 mm in diameter. After 48 hours, surface colonies somewhat flattened, 3.5 to 4.0 mm in diameter; deep colonies ellipsoidal and biconvex with hemolytic area 2.5 to 3.0 mm in diameter. Blood agar slants : After 24 hours at 38° C. , heavy, viscid, grayish white growth. Coagulated serum: Liquefaction. Broth: Slow growth; slight turbidity; considerable sediment. Litmus milk: Alkaline. Partial coagula- tion. Potato: No growth. Indole not produced. No acid from glucose or other carbohy- drates. Nitrites not produced from nitrates. Aerobic. Optimum temperature, 36° C. Killed at 58° to 59° C. in five minutes. Not pathogenic for laboratory animals. Source: Isolated from acute cases of ophthalmia (pink eye) of cattle. Genus VIII. Noguchia Olilsky et al., 1934.* (Olitsky, Syverton and Tyler, Jour. Exp. Med., 60, 1934, 382.) No.gu'chi.a. M.L. noun Noguchia named for Hideyo Noguchi, the bacteriologist who isolated the type species of this genus. Small, slender rods. Encapsulated. Motile by means of peritrichous flagella. Gram-nega- tive. Produce a mucoid type of growth which, on initial isolation, occurs with some diffi- culty on ordinary media. Aerobic to facultatively anaerobic. Optimum temperature, be- tween 28° and 30° C. Found in the conjunctiva of man and animals affected by a follicular type of disease. The type species is Noguchia granulosis (Noguchi) Olitsky et al. Ketj to the species of genus Noguchia. I. Produce acid from carbohydrates. A. Produces acid from raffinose, maltose and salicin. 1. Noguchia granulosis. B. Does not produce acid from raffinose, maltose or salicin. 2. Noguchia simiae. II. Does not produce acid from carbohydrates. 3. Noguchia cxmiculi. 1. Noguchia granulosis (Noguchi, 1928) Olitsky et al., 1934. {Bacterium granulosis Noguchi, Jour. Exp. Med., 48, 1928, Supple- ment 2, 21; Olitsky, Syverton and Tyler, Jour. Exp. Med., 60, 1934, 382.) gra.nu.lo'sis. L. noun granulum a small grain; Gr. suffix -osis; M.L. fem.n. granulosis pathological condition characterized by col- lection of granules; M.L. gen. noun granulo- sis of granulosis. Rods measuring 0.25 to 0.3 by 0.8 to 1.2 microns in young cultures; involution forms occur in old cultures on blood agar. Motile by means of a single flagellum which usually arises from one of the poles but which occa- sionally appears as if attached to one side. Gram-negative. No growth on plain agar or broth. Blood agar colonies: Minute, round, shiny, somewhat raised, almost transparent or slightly grayish in 48 hours; later the colonies increase in size and are grayish, opalescent and somewhat sticky. Old col- onies have a brownish or yellowish tint. Semi-solid Leptospira medium: Grayish white, diffuse growth forming a delicate zone 1 cm deep. Liquid Leptospira medium: Diffuse, Arranged by Prof. C. D. Kelly, McGill University, Montreal, P.Q., Canada, October, 1938.
