Source: Seven strains were isolated from human feces. Habitat: Probably the intestinal canals of mammals. 9. Sphaerophorus niortiferus (Harris, 1901) Prevot, 1938. {Bacillus mortiferus Harris, Jour. Exp. Med., 6, 1901, 519; Prevot, Ann. Inst. Past., 60, 1938, 299.) mor.ti'fer.us. L. noun mors death; L. gen. noun mortis of death; L. v. fero to bear; ALL. adj. mortiferus death-bearing. Rods, 0.6 bj' 2.0 microns, with rounded ends, occurring singly, in pairs or in short chains, with filamentous and pleomorphic forms, some showing metachromatic gran- ules. Non-motile. Gram-negative. Serum or ascitic fluid is required for growth. Hydrocele gelatin: No liquefaction. No gas. Hydrocele agar: Deep colonies small, ir- regular, translucent, granular, light brown. Surface colonies small, regular or fringed, 1 to 2 mm in diameter, yellowish brown; fecal odor. Glucose-human blood agar: Gas with a disagreeable odor; browning of the hemo- globin. Hydrocele broth: Growth with heavy, viscous, whitish deposit and foul-smelling gas. Hj^drocele milk: Acid but no coagulation. In 6 days the milk is peptonized. Indole produced in hydrocele broth. Hydrogen sulfide produced in hydrocele broth. Anaerobic. Optimum temperature, 37° C. Pathogenic for guinea pigs, rabbits and mice. Source: Isolated from a liver abscess of man. Habitat : Presumabl}^ found in various in- fections in man. 10. Sphaerophorus gonidiaformans (Tunnicliff and Jackson, 1925) Prevot, 1938. (Bacillus gonidiaformans Tunnicliff and Jackson, Jour. Inf. Dis., 36, 1925, 430; Actinomyces gonadiformis (sic) Bergey et al., Manual, 3rd ed., 1930, 469; Bacteroides goni- diaformans Hauduroy et al.. Diet. d. Bact. Path., 1937, 62; Prevot, Ann. Inst. Past., 60, 1938, 299.) go.ni.di.a.for'mans. Gr. noun gone off- spring, seed; M.L. noun gonidium gonidium; L. part. adj. formans forming; M.L. adj. gonidiaformans gonidia-forming. Rods, 0.5 by 1.0 to 3.0 microns, with rounded ends; gonidia form within the rods, developing into short or long, wavy filaments. Non-motile. Show bipolar stain- ing; also show red granules with Giemsa stain. Gram-negative. Serum or ascitic fluid is required for growth. Gelatin: No liquefaction. No growth. Blood agar colonies: Thick,whitish, moist, entire. Glucose media: Slight gas produced. Broth: Slight development; flocculent sediment. Litmus milk: No growth. Potato: No growth. No acid or gas from glucose, salicin, man- nitol or inulin. Anaerobic. Optimum temperature, 37° C. Pathogenic for rabbits and guinea pigs. Source : Isolated from tonsils. Habitat: Presumably the pharynx. 11. Sphaerophorus freundii (Haudu- roy et al., 1937) Prevot, 1938. (Freund, Cent, f. Bakt., I Abt., Ong., 88, 1922, 9; Bacterium of Freund, Weinberg et al., Les Microbes Anaerobies, 1937, 706; Bacteroides freundii Hauduroy et al., Diet. d. Bact. Path., 1937, bl ; Sphaerophorus freundi (sic) Prevot, Ann. Inst. Past., 60, 1938, 299.) freun'di.i. M.L. gen. noun freundii of Freund; named for F. Freund, the bacteri- ologist who first described this species. Short, ellipsoidal rods, 0.3 to 0..35 by 0.8 to 1.0 micron, occurring singly, in pairs and in chains. Pleomorphic in cultures with ir- regular filaments and swollen forms. Non- motile. Show bipolar staining. Gram- negative. Serum or ascitic fluid is required for growth. Gelatin: No liquefaction. Ascitic agar: Deep colonies lenticular in 1 to 3 days, small, yellowish.
