Serum glucose agar: Deep colonies len- ticular. Gas produced. Glucose agar: Surface colonies pinhead, dew-drop, opaque, yellowish. Glucose serum broth: Turbidity; thin fiocculent growth. Gas produced with fetid odor. Serum milk: Coagulated in 3 daj-s. Not peptonized. Indole is produced in glucose broth. Hydrogen sulfide is produced in glucose broth. Coagulated hydrocele fluid not liquefied. Acid and gas from glucose, maltose, su- crose and inannitol. I']thanol and butyric and lactic acids are produced from carbohy- drates. Anaerobic. Optimum temperature, 37° C. Pathogenic for rabbits and guinea pigs. Source: One strain was isolated from a purulent meningitis following otitis in man. Habitat: Found in various infections in man. 12. Sphaerophorus pyogenes (Haudu- roy et al., 1937) Prevot, 1938. (Buday, Cent, f. Bakt., I Abt., Orig., 77, 1916, 453; Bacillus pyogenes anaerobins Bela-Johan, Cent. f. Bakt., I Abt., Orig., 87, 1922, 290; not Bacillus pyogenes anaerobius Kruse, in Fliigge, Die Mikroorganismen, 3 Aufl., 2, 1896, 244; Bacteroides pyogenes Hauduroy et al.. Diet. d. Bact. Path., 1937, 69; Prevot, Ann. Inst. Past., 60, 1938, 299.) py.o'ge.nes. Gr. noun pyum pus; Gr. v. gennaio to produce; M.L. adj. pyogenes pus- producing. Short, ellipsoidal rods, occurring singly, in short chains and as pleomorphic, filamen- tous forms. Non-motile. Show bipolar stain- ing. Gram-negative. Serum or ascitic fluid is required for growth; isolation in culture media is easier after animal passage. Serum agar: Deep colonies small, puncti- form. Some gas produced. Surface colonies fine, transparent, with flat borders, 0.5 mm in diameter. Ascitic broth: The optimum concentra- tion of ascitic fluid is from 30 to 50 per cent. Fiocculent growth with a foamy layer on the surface. Gas produced. Tarozzi broth with liver: Rapid growth with turbidity and later a fiocculent deposit. Foamy layer on the surface. Hydrogen sulfide not produced. Anaerobic. Optimum temperature, 37° C. Pathogenicity: Pathogenic for rabbits and guinea pigs when pus from a lesion is in- jected. Cultures other than those in ascitic broth show no pathogenicity. Source: Isolated from abscesses of the liver and lungs following septic war wounds; also isolated from the blood stream follow- ing tonsillectomies. Habitat: Found in various infections in man. 13. Sphaerophorus influenzaeforniis (Russ, 1905) Prevot, 1938. (Bacillus influen- zaeformis Russ, Cent. f. Bakt., I Abt., Orig., 39, 1905, 357; Bacteroides russii Hau- duroy et al.. Diet. d. Bact. Path., 1937, 73; Prevot, Ann. Inst. Past., 60, 1938, 299.) in.flu.en.zae.for'mis. M.L. noun iyiflu- enza influenza; L. noun forma shape; M.L. adj. influenzaeformis influenza-like. Short rods, 0.5 by 1.5 microns, with rounded ends ; some strains are pleomorphic with involution forms and swollen fila- ments. Non-motile. Show bipolar staining. Gram-negative. Gelatin: No liquefaction. Agar: Deep colonies lenticular, sometimes surrounded by a number of smaller colonies. No gas. Glucose agar: Surface colonies semi- transparent, dew-drop, grayish. Peptone broth: No growth. Glucose broth: Uniform turbidity which deposits as a granular mass. No gas pro- duced. Milk: Slow growth with coagulation in several weeks. No gas or digestion of coagu- lum. Indole produced in glucose broth. Hydrogen sulfide produced in glucose broth. Anaerobic. Optimum temperature, 37° C. Pathogenicity: One strain was patho- genic for rabbits, mice and guinea pigs. Source: One strain was isolated from a
