Page:Bergey's manual of determinative bacteriology.djvu/519

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FAMILY IX. BREVIBACTERIACEAE
497

Source: Isolated from a triturated speci- men of the mud-dauber wasp, Sceliphron cementariiim Dru. Habitat: Unknown.

10. Brevibacteriuni sociovivuni (ZoBell and Upham, 1944) Breed, 1953. {Bacterium sociovivnm ZoBell and Upham, Bull. Scripps Inst. Oceanography, La Jolla, 5, 1944, 269; Breed, Riassunti delle Comunicazioni, VI Congresso Internaz. di Microbiol., Roma, 1, 1953, 14; also see Atti del VI Congresso Internaz. di Microbiol., Roma, 1, 1955, 14.) so.ci.o.vi'vum. L. noun socius com- panion, partner; L. v. vivo to live; M.L. adj. sociovivus partner living. Rods, 0.5 to 0.8 by 3.0 to 4.0 microns, with rounded ends, occurring singly, inpairs and in chains. Non-motile. Gram-positive, but tends to destain, leaving Gram-positive cell wall and granules. All differential media except the fresh- water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: Irregular, sunken, gray- ish white; filamentous margin. Gelatin stab: Crateriform liquefaction becoming stratiform. Agar colonies: 2 to 4 mm in diameter, circular, convex, smooth, entire, darker center. Agar slant: Luxuriant, beaded, glisten- ing, butyrous growth with no pigment. Sea-water broth: No pellicle; no turbid- ity; heavy, flocculent sediment. Fresh-water broth: Fair growth. Litmus milk: Decolorized; neutral; com- pletely peptonized in 20 days. Potato: Abundant, dull, light cream- colored growth. Potato darkened. Indole not produced. Hydrogen sulfide not produced. Acid but no gas from glucose, maltose and mannitol. No acid from glycerol, lactose, sucrose or salicin. Starch is hydrolyzed. Non-lipolytic. Nitrites not produced from nitrates. Ammonia produced from peptone but not from urea. Casein is digested. Aerobic, facultatively anaerobic. Optimum temperature, between 20° and 25° C. Source: Found associated with sedentary organisms in the sea. Habitat: Commonly found on submerged surfaces and on sessile diatoms in sea water.

11. Brevibacterium immotum (ZoBell and Upham, 1944) Breed, 1953. {Bacterium immotum ZoBell and Upham, Bull. Scripps Inst. Oceanography, 5, 1944, 271; Breed, Riassunti delle Comunicazioni, VI Con- gresso Internaz. di Microbiol., Roma, 1, 1953, 14; also see Atti del VI Congresso Internaz. di Microbiol., Roma, 1, 1955, 14.) im.mo'tum. L. adj. immotus motionless. Rods, 0.8 by 3.1 to 8.6 microns, with rounded ends, occurring singly, in pairs and in long chains. Non-motile. Gram-positive, but tends to destain, leaving Gram-positive outline and granules. All differential media except the fresh- water broth, litmus milk and potato were prepared with sea water. Gelatin colonies: Small, circular, raised, gray, slowly digest gelatin. Gelatin stab : Crateriform liquefaction be- coming infundibuliform. Beaded growth along line of stab. No pigment. Agar colonies: 1 to 2 mm in diameter, circular, convex, smooth, lobate margin, darker centers. Agar slant: Luxuriant, glistening, echinu- late, mucoid growth with no pigment. Sea-water broth: No pellicle; moderate turbidity; abundant, flocculent sediment. Fresh-water broth: Scant growth. Litmus milk: Decolorized; neutral; partly peptonized in 20 days. Potato: Luxuriant, mucoid, cream}^ growth which darkens potato. Indole not produced. Hydrogen sulfide not produced. Acid but no gas from glucose, maltose, xylose and mannitol. No acid from glycerol, lactose, sucrose or salicin. Starch is hydrolyzed. Non-lipolytic. Nitrites not produced from nitrates. Ammonia produced from peptone but not from urea. Casein is digested.