Page:Bergey's manual of determinative bacteriology.djvu/615

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FAMILY XII. CORYNEBACTERIACEAE
593

Source: Isolated from slimy heads of Dac- tylis glomerata. Habitat: Pathogenic on Dactylis glomerata. 23. Corynebacteriuni agropyri (C- Gara, 1916) Burkholder, 1948. {A planohader agropyri O'Gara, Phytopath., 6, 1916, 343; Burkholder, in Manual, 6th ed., 1948, 395.) ag.ro. py 'ri . Or. noun agrus field ; Or. noun pyrus wheat; M.L. neut.n. Agropyron ge- neric name of a grass; M.L. gen. noun agro- pyri of Agropyron. Rods 0.4 to 0.6 by 0.6 to 1.1 microns. En- capsulated. Non-motile. Gram-variable. Gelatin: No liquefaction. Nutrient agar slant: Meager, yellow, very viscid growth. Broth: Light clouding with yellow precipi- tate. Milk: Little-changed. Yellow sediment formed. Acid but no gas from glucose, lactose, sucrose and glycerol. Starch: Hydrolysis feeble. Nitrites produced from nitrates. Optimum temperature, between 25° and 28° C. This species is very similar to and may be identical with Corynebacterium rathayi Dowson. Source: Isolated from slimy heads of wheat grass. Habitat: Found on wheat grass, Agro- pyron smithii. 24. Corynebacterium fascians (Til- ford, 1936) Dowson, 1942. (Phytomonas fas- cians Tilford, 54th Rept. Ohio Agr. Exp. Sta. Bull. 561, 1936, 39; also see Jour. Agr. Res., 53, 1936, 393; Dowson, Trans. Brit. Myc. Soc, 25, 1942, 313.) fas'ci.ans. L. part. adj. fascians banding, binding. Rods 0.5 to 0.9 by 1.5 to 4.0 microns. Non- motile. Gram-positive. Gelatin: No liquefaction. Potato-glucose agar colonies: Light cream-colored colonies appear after 72 hours. Punctiform, circular, later cadmium- yellow to deep chrome. Nutrient agar slant : After one week streak is filiform, flat, dull to glistening, smooth, opaque, cream-colored and butyrous. Broth: Slightly turbid. Fragile pellicle with distinct rim. Milk: Litmus becomes blue. Other changes slight . Indole not produced. Hydrogen sulfide produced. Acid but no gas from glucose, galactose, fructose, mannose, arabinose, xylose, malt- ose, sucrose, glycerol, mannitol and dextrin. No acid from rhamnose, lactose, raffinose or inulin. Starch not hj-drolyzed. Nitrites produced from nitrates. Grows in 8 per cent salt. Aerobic. Optimum temperature, between 25° and 28° C. Source: Described from 15 single-cell iso- lates from fasciated growths on sweet peas. Habitat: Pathogenic on sweet pea, chrys- anthemum, geranium, petunia, tobacco, etc. 25. Corynebacterium hypertrophicans (Stahel, 1933) Burkholder, 1948. (Pseudo- nionas hypertrophicans Stahel, Phyt. Ztschr., 6, 1933, 445; Burkholder, in Manual, 6th ed., 1948, 398.) hy. per. tro 'phi. cans. Gr. pref. hyper very; Gr. adj. trophicus well-fed, stout, over- grown; M.L. adj. hypertrophicans becoming overgrown, producing a hypertrophy. Rods 0.6 to 0.8 by 1.2 to 2.8 microns. Mo- tile by means of a single polar flagellum. Bipolar staining. Gram-positive. Gelatin: No growth. Agar colonies: Slow growing, circular, raised, wet-shining, white. Broth plus sucrose: Growth good. No pellicle. Milk: No visible change. Indole not produced. Hydrogen sulfide not produced. Acid but no gas from glucose, fructose and sucrose. No acid from lactose and glyc- erol. The acids from sucrose are lactic and formic. Nitrites not produced from nitrates. Aerobic. Source: Isolated from witches' brooms. Habitat: Pathogenic on Eugenia latifolia. 26. Corynebacterium poinsettiae Starr