Gelatin: No liquefaction. Gelatin deep colonies: Small, gray, fila- mentous. Agar deep colonies: Irregular, gray, trans- lucent, filamentous. Broth: Uniformly turbid. Litmus milk: Acid, but no further change. Potato: Gray, filamentous growth; sub- stance not digested. Acid and gas from glucose and lactose. Acid but no gas from sucrose and dulcitol. Starch not fermented. Coagulated albumin: No liquefaction. Anaerobic. Growth occurs at 22° C. in gelatin. Source: Isolated from human feces. Habitat: Not determined. 47. Clostridium sartagoformum Par- tansky and Henry, 1935. (Jour. Bact., 30, 1935, 570.) sar.ta.go.for'mum. L. noun sartago, -inis a frying pan; L. noun forma shape; L. adj. formus warm; M.L. adj. sartagoformum (probably intended to mean) shaped like a frying pan. Slender, curved rods, 0.3 to 0.5 by 3.5 to 6.0 microns, with rounded ends, occurring singly. Spores ovoid, terminal, swelling the cells. Motile. Gram-positive. Gelatin: No liquefaction. Agar surface colonies (anaerobic) : Con- vex, discrete, circular, transparent to white and opaque. Surface moist and smooth. Agar deep colonies: Regular, lenticular, smooth. Broth: No growth. Clear. Glucose broth: Turbid; gas bubbles. Litmus milk: Acid; slowly coagulated withsome gasproduction. Clot not digested. Potato: Very scant growth. No gas in sur- rounding liquid. Indole not produced. Acid and gas from xylose, glucose, fruc- tose, galactose, sucrose, lactose, maltose, raffinose, inulin, salicin, mannitol, acetate and butyrate. Starch, ethanol, glycerol and dulcitol not fermented. Nitrites not produced from nitrates. Coagulated albumin: No liquefaction. Blood agar: No hemolysis. Blood serum: No liquefaction. Scant growth. Brain medium: No blackening or diges- tion. Some gas is produced. Anaerobic. Optimum temperature, 37° C. Distinctive character: Ferments sulfite- waste liquor in 40 per cent concentration, producing butyric and acetic acids, H2 and CO2. Source: Isolated from garden soil and from stream and lake mud. Habitat: Presumably soil. 48. Clostridium paraputrificum (Bien- stock, 1906) Snyder, 1936. (Art V, Bienstock, Fortschr. d. Med., 1, 1883, 612; Bacillus diaphthirus Trevisan, I generi e le specie delle Batteriacee, 1889, 15; Bacillus paraputrificus Bienstock, Ann. Inst. Past., 20, 1906, 413; also see Strassburger Med. Zeit., 3, 1906, 111 ; Snyder, Jour. Bact., 32, 1936, 401.) pa.ra.pu.tri'fi.cum. Gr. pref. para beside, by; M.L. noun putrijicum a specific epithet; M.L. adj. parapw^rz^cws resembling (Clostri- diuvi) putrificum. Description taken from Hall and Synder (Jour. Bact., 28, 1934, 181). Straight or slightly curved rods, 0.3 to 0.5 by 2.0 to 6.0 microns, with rounded ends, occurring singly, in pairs or in short chains. Spores ovoid, terminal, swelling the cells. Motile by means of peritrichous flagella. Gram-positive. Gelatin : No liquefaction. Gas is produced. Agar deep colonies: Small, irregular, opaque, dense, cottony masses. Gas is pro- duced. Blood agar surface colonies (anaerobic) : Delicate, irregular, round- topped dew- drops. No hemolysis. Broth: Diffuse turbidity. Milk: Usually coagulated in from 6 to 10 days. Abundant gas, but no peptonization. Indole not produced. Acid and gas from glucose, fructose, galac- tose, maltose, lactose, sucrose, raffinose, dextrin, soluble starch, amygdalin and salicin. Xylose, inulin, mannitol and glyc- erol not fermented. Nitrates reduced (Reed, Jour. Bact., 44 1 1942,425). Coagulated albumin: No liquefaction. Blood serum: No liquefaction or dis- coloration.
