Brain medium: No blackening or diges- tion. Non-proteolytic. Anaerobic. Grows well at 37° C. Not pathogenic for guinea pigs or rabbits. Source: Isolated from feces and gaseous gangrene and from post-mortem fluid and tissue cultures. Habitat : Presumably occurs commonly in the intestinal canals of human beings. 49. Clostridium indologenes (Prevot, 1948) McClung and McCoy, comb. nov. (Pleciridium indologenes Prevot, Ann. Inst. Past., 74, 1948, 165.) in.do.lo'ge.nes. M.L. neut.n. indolum indole; Gr. v. gennaio to produce; M.L. adj. indologenes indole-producing. Straight rods, 0.6 by 3.0 to 4.0 microns, occurring singly or in pairs. Spores rare, ovoid, large-sized and distinctly terminal. Motile in very young cultures. Gram-posi- tive. Gelatin: No liquefaction. Agar deep colonies: Lenticular with sec- ondary off -shoots; little gas produced, if any. Peptone broth: Turbid. Glucose broth: Abundantly turbid; vis- cous sediment; putrid odor. Milk: No change. Indole produced. Hydrogen sulfide produced. Glucose, lactose and galactose are fer- mented . Nitrites not produced from nitrates. Coagulated proteins: Not attacked. Anaerobic. Optimum temperature, 37° C. Optimum pH, 7.8. Not pathogenic for guinea pigs. Source: Isolated from a sample of "poto- poto" in Africa. Habitat: Not determined. 50. Clostridium cochlearium (Bulloch et al., 1919) Bergey et al., 1923. {Bacillus Type IIIc, Mcintosh, Med. Res. Counc, Spec. Rept. Ser. No. 12, 1917, 20; Bacillus cochlearius Bulloch, Bullock, Douglas, Henry, Mcintosh, O'Brien, Robertson and Wolf, Med. Res. Counc, Spec. Rept. Ser. No. 39, 1919, 40; Bergey et al., Manual, 1st ed., 1923, 333.) coch.le.a'ri.um. L. noun coclear (cochlear) a spoon; M.L. adj. cochlearius spoon-like. Straight, slender rods, occurring chiefly singly and infrequently in pairs or short chains. Spores ovoid, terminal, swelling the cells. Motile by means of peritrichous flagella. Weakly Gram-positive. Gelatin: No liquefaction. Agar surface colonies (anaerobic) : Circu- lar, clear, entire or crenated. Agar deep colonies: Lenticular, entire. Broth: Turbid. Litmus milk: Unchanged. Glucose and other carbohydrates not fermented. Coagulated albumin: No liquefaction. Blood serum: No liquefaction. Brain medium: No blackening or diges- tion. Meat medium: Slightly reddened. No blackening or digestion. Little gas produced of non-putrefactive odor. Anaerobic. Optimum temperature, between 30° and 35° C. Not pathogenic. Source : Isolated from human war wounds and septic infections. Habitat: Not determined. Probably oc- curs in soil. 51. Clostridium kluyveri Barker and Taha, 1942. {Clostridium kluyverii (sic) Barker and Taha, Jour. Bact., 43, 1942, 347.) kluy've.ri. M.L. gen. noun kluyveri of Kluyver; named for Prof. A. J. Kluyver of Delft, Holland, in whose laboratory this organism was discovered. Straight to slightly curved rods, 0.9 to 1.1 by 3.0 to 11.0 microns, usually occurring singly and in pairs, occasionally in long chains. Spores ovoid, 1.3 by 1.8 microns, terminal, swelling the cells. Motile by means of peritrichous flagella. Generally Gram- negative; some strains are weakly Gram- positive when young. Iron-gelatin (Spray) : No growth. Agar surface colonies (anaerobic) : Growth slow and restricted by residual traces of
