Optimum temperature, 37° C. Grows well at room temperature. Neither toxin nor hemolysin produced. Not pathogenic for guinea pigs or rabbits. Source: Isolated from a human cadaver and from the peritoneum of a rabbit. Habitat: Not determined. 55. Clostridium saprogenes (Salus, 1904) McClung and McCoy, comb. nov. (Bacillus saprogenes carnis Salus, Arch. f. Hyg., 51, 1904, 114; Bacillus saprogenes Salus, ibid., 115; Plectridium saprogenes Prevot, Ann. Inst. Past., 61, 1938, 87; also see Prevot and Weislitz, Ann. Inst. Past., 72, 1946, 444.) sap.ro'ge.nes. Gr. adj. saprus rotten; Gr. V. gennaio to produce; M.L. part. adj. saprogenes rot-producing. Rods, 1.5 by 8.0 microns, curved, in chains and filaments. Spores ovoid, terminal. Mo- tile. Gram-positive. Gelatin: Slow liquefaction. Agar deep colonies: Lenticular or spheri- cal; gas is produced. Liquid media: Gas production; disagree- able odor. Glucose broth: Turbid; sediment; pene- trating, putrid odor due in part to hydrogen sulfide. Milk: Coagulated with a disagreeable odor. Glucose, fructose, maltose, galactose, su- crose, lactose, arabinose, xylose, sorbitol, dulcitol, inulin and starch are fermented. Glycerol and mannitol are slightly attacked. Nitrites not produced from nitrates. Coagulated egg, serum, fibrin and brain: Not attacked. Anaerobic. Optimum temperature, 37° C. Not pathogenic. Source: Isolated from spoiled meat and from an industrial fermentation sample. Habitat: Probably soil. 56. Clostridium perenne (Prevot, 1940) McClung and McCoy, comb. nov. (Acuformis perennis Prevot, Compt. rend. Soc. Biol., Paris, 133, 1940, 576.) per.en'ne. L. adj. perennis lasting through the year or through many years, perennial. Straight rods, 0.3 to 0.4 by 1.6 to 3.0 microns for non-sporulating cells and 0.5 by 3.0 to 5.0 microns for sporulating cells, with rounded ends, occurring in pairs or in chains of 3 to 16 cells. Spores ovoid, termi- nal, measuring 0.6 by 1.0 micron. Non- motile. Gram-positive. Gelatin: No liquefaction. Agar deep colonies: Lenticular, whitish, 1 to 2 mm in diameter. Agar is split by gas. Glucose broth: Abundantly turbid; gas liberated has a slight, but not disagreeable, odor of volatile acids. Peptone broth: Abundantly turbid; no gas. Milk: Rapidly acidified, then coagulated with liberation of gas and retraction of clot. Indole not produced. Hydrogen sulfide not produced. Glucose, fructose, maltose, galactose, sucrose, arabinose and lactose are strongly fermented. Nitrites not produced from nitrates. Anaerobic. Optimum temperature, between 33° and 37° C. Optimum pH, about 7.0. Not pathogenic for guinea pigs, mice or rabbits. Source: Isolated from a case of chronic appendicitis. Habitat: Not determined. 57. Clostridium thermosaccharolyti- cum McClung, 1935. (Jour. Bact., 29, 1935, 200.) ther.mo.sac.cha.ro.ly'ti.cum. Gr. adj. thermus hot; Gr. noun saccharum sugar; Gr. adj. lyticus dissolving; M.L. adj. thermo- saccharolyticus (presumably intended to mean) thermophilic and sugar-fermenting. Slender, granulated rods, 0.4 to 0.7 by 3.5 to 7.5 microns, occurring singly and in pairs, not in chains. Spores spherical, terminal, swelling the cells. Motile by means of peri- trichous flagella. Gram-negative. Gelatin: No liquefaction. Pea-infusion agar surface colonies (anae- robic) : Granular, grayish white, raised center, feathery edges. Glucose-tryptone agar deep colonies: Small, lenticular, smooth. Liver-infusion broth over liver meat: Turbid; gas produced.
