Litmus milk: Litmus reduced. Acid and slow but firm coagulation; coagulum split with gas. Clot not digested. Indole not produced. Acid and gas from arabinose, fructose, galactose, glucose, mannose, xylose, cello- biose, lactose, maltose, sucrose, trehalose, dextrin, glycogen, maize starch, amygdalin, esculin, alpha-methyl-glucoside and salicin. Raffinose weakly fermented. Rhamnose, inulin, pectin, erythritol, inositol, mannitol, glycerol, quercitol and Ca-lactate not fer- mented. Sorbitol and dulcitol not fermented (Mercer and Vaughn, Jour. Bact., 6£, 1951, 27). Cellulose not fermented. Nitrites not produced from nitrates. Coagulated albumin: No liquefaction. Blood serum: No liquefaction. Brain medium: No blackening or diges- tion. Meat medium: No blackening or diges- tion. Anaerobic. Optimum temperature, between 55° and 62° C. Thermophilic. Not pathogenic on feeding to white rats or on injection to rabbits. Distinctive character: Differentiated from Clostridium tariarivorum Mercer and Vaughn by not fermenting tartrate. Source: Isolated from hard-swell of canned goods and from soil. Habitat: Not determined. 58. Clostridium indolis McClung and McCoy, nom. nov* {Terminosporus indolo- genes Bezjak, Ann. Inst. Past., 82, 1952, 101.) in'do.lis. M.L. neut.n. indolum indole; M.L. gen. noun indolis of indole. Straight to slightly curved rods, 0.6 by 3.0 to 4.0 microns. Spores terminal, round, measuring 1.0 to 1.5 microns. Motile. Gram- negative. Gelatin: No growth. Veillon agar colonies: Small, lenticular; no gas. Blood agar colonies : After 3 days incuba- tion, 2 mm in diameter, moist, grayish, round, irregular; there is also a spreading tendency. Glucose broth: Uniformly turbid. Milk: Acid and coagulated in 2 days; gas. Indole is produced. Hydrogen sulfide is produced. Acid and gas from glucose, sucrose, maltose and lactose. Mannitol not fer- mented. Coagulated proteins: Not attacked. Anaerobic. Grows well at 37° C. but not at ordinary temperatures nor at 46° C. Pathogenicity: Mice injected peritoneally show no efi'ects. Source: Isolated from a patient operated on for cancer of the large intestine. Habitat: Probably the human intestine. 59. Clostridium caloritolerans Meyer and Lang, 1926. (Jour. Inf. Dis., 39, 1926, 321.) ca.lo.ri.to'le.rans. L. noun calor heat; L. part. adj. tolerans tolerating; M.L. caloritolerans heat-tolerating. Rods, 0.5 to 0.8 by 8.0 to 10.0 microns, with rounded ends, occurring singly, in pairs, in chains and in curved filaments. Spores spherical or pear-shaped, terminal, swelling the cells. Motile by means of peri- trichous flagella. Gram-positive. Gelatin: No liquefaction. Liver agar deep colonies: Small, flat, transparent discs with large, polar tufts. Some colonies become fluffy. Glucose blood agar surface colonies (anaerobic): Small, flat, grayish, rhizoidal. No hemolysis. Broth: Slightly turbid. Glucose broth: Abundantly turbid; clear- ing by sedimentation; gas is produced. Brom cresol purple milk: No change. Indole not produced. Acid and gas from glucose, galactose and maltose. Fructose feebly fermented. Lac- tose, sucrose, raffinose, inulin, salicin, mannitol, inositol and glycerol not fer- mented.
- The specific epithet indolis is used here in lieu of indologenes because the latter is pre-
occupied in the genus Clostridium for an earlier-described organism (see species number 49, Clostridium indologenes McClung and McCoy).
