Synthetic glucose agar: Thin, flat, almost black growth with greenish tinge. Potato agar: Vinaceous, tawny colored growth producing a vinaceous tawny soluble pigment. Glycerol synthetic solution: Poor, flaky growth at bottom. Glucose broth: Numerous minute, whitish colonies at bottom of container. Milk: Very slight growth. Not coagulated; not peptonized. Potato: Restricted, raised, pinkish growth. Aerial mycelium scant, white. Soluble, gray to brown pigment. Starch is hydrolyzed. Tyrosinase reaction: Negative. Nitrites not produced from nitrates. Grows well under anaerobic conditions. Grows well at 37.5° C. Source: Isolated from sour soil. Habitat: Soil. 134. Streptoniyces coroniformis (Mil- lard and Burr, 1926) Waksman, 1953. (.4c- tinomyces coroniformis Millard and Burr, Ann. Appl. Biol., 13, 1926, 601; Waksman, in Waksman and Lechevalier, Actinomy- cetes and Their Antibiotics, Baltimore, 1953, 114.) co.ro.ni.for'mis. L. fem.n. corona crown; L. ievti.n. forma form; M.L. adj. coroniformis crown-shaped. Aerial mycelium: Simple branching. Spores ellipsoidal, 0.6 by 0.8 micron. Gelatin: Fair growth. Weak or no lique- faction. Synthetic sucrose agar: Gray to greenish discrete colonies partially coalescing. White aerial mycelium covering edges of growth. Synthetic glucose agar: Grayish discrete colonies growing into medium weakly pro- duced. No aerial mycelium. Potato agar: Wrinkled, grayish growth. No aerial mycelium. Glycerol synthetic solution: Very poor growth in form of small flakes at bottom. Glucose broth: Fair growth at bottom. Milk: A few colonies on surface. Not co- agulated; weakly peptonized. Potato: Raised, grayish growth. Aerial mycelium white. Plug pigmented brownish around and under growth. Starch may be weakly hydrolyzed. Tyrosinase reaction: Negative. Nitrites weakly produced from nitrates. Fair growth at 37.5° C. Source: Isolated from peat soil. Habitat: Soil. 135. Streptoniyces spiralis (Millard and Burr, 1926) Waksman, 1953. [Actinomyces spiralis Millard and Burr, Ann. Appl. Biol., 13, 1926, 601; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 114.) spi.ra'lis. Gr. fem.n. spira a spiral; M.L. adj. spiralis like a spiral. Vegetative growth: Lichenoid, smooth, yellowish golden growth. No soluble pig- ment . Aerial mycelium: Sporophores straight or spiral-forming. Spores cylindrical, 0.9 by 1.0 to 1.7 microns. Gelatin: Limited, gray growth. Aerial mycelium scant, white. Rapid liquefaction. Synthetic sucrose agar: Rough or granu- lar growth. Aerial mycelium vinaceous buff to dark grayish olive. Soluble, pale vina- ceous-f awn-colored pigment. Synthetic glucose agar: Fairly rough, pale gray growth. Aerial mycelium scant, white. Soluble, j'ellowish to pink pigment. Potato agar: Granular, gray growth. Aerial mycelium abundant, white to olive- buff. Soluble, light golden brown pigment. Glycerol synthetic solution: No growth. Glucose broth: Minute compact colonies at bottom and on surface. Aerial mycelium scant, white. Milk: Good surface growth covered wdth abundant, white aerial mycelium. Coagu- lated, rapidly peptonized. Potato: Poor, wrinkled, grayish vina- ceous growth. Aerial mycelium w^hite to grayish vinaceous. Plug colored brown around and below growth. Starch is not hydrolyzed. Tyrosinase reaction: Negative. Nitrites not produced from nitrates. Does not grow at 37.5° C. Source: Isolated from a heap of decaying grass. Habitat: Found in grass compost so far as known. 136. Streptoniyces sampsonii (Millard
