Page:Bergey's manual of determinative bacteriology.djvu/835

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FAMILY III. STREPTOMYCETACEAE
813

and Burr, 1926) Waksman, 1953. (Actino- myces sampsonii Millard and Burr, Ann. Appl. Biol., IS, 1926, 601; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 115.) samp.so'ni.i. M.L. gen. noun sampsonii of Sampson; named for a person, Sampson. Original description supplemented by observations made bj^ Waksman and Gor- don. Aerial mycelium: Long branching aerial hyphae. Spores cylindrical, 0.5 by 0.8 to 1.0 micron. Spores ellipsoidal to spherical (Waksman and Gordon). Gelatin: Scant, gray surface growth. A trace of whitish aerial mycelium. Rapid liquefaction. Synthetic sucrose agar: Good, wrinkled, pale gray to white growth. Aerial mycelium very scant, white. Soluble, green to buff pigment. Synthetic glucose agar: Good, wrinkled, white to gray growth. Aerial mycelium scant, white. Soluble, yellow to brownish pigment. Glycerol synthetic solution: No growth. Glucose broth: Good surface and bottom growth as well as many colonies clinging to side of tube. Aerial mycelium white. Milk: Good, whitish surface growth. No aerial mycelium. Not coagulated; not pep- tonized. Rapid peptonization (Waksman and Gordon). Potato: Wrinkled, grayish growth. Aerial mj^celium white. Soluble, golden brown pigment. None observed (Waksman and Gordon). Starch is not hydrolyzed. Starch is rap- idly hjdrolyzed (Waksman and Gordon). Tyrosinase reaction: Negative. Nitrites produced from nitrates. Optimum temperature, 28° C. Source: Isolated from a medium-sized, ruptured potato scab. Habitat: Unknown. 137. Slreploniyces inlerinediiis (Krii- ger, 1890, emend. Wollenweber, 1920) Waks- man, 1953. (Oospora intermedia Kriiger, Berichte der Versuchsstat. f. Zuckerrohrs, Kergok-Legal, 1890; Actinomyces inter - medius Wollenweber, Arb. d. Forschungs- inst. fiir Kartoffelbau, 1920, 16; Waksman, in Waksman and Lechevalier, Actinomy- cetes and Their Antibiotics, Baltimore, 1953, 116.) in.ter.me'di.us. L. adj. intermedius in- termediate. Vegetative growth: Green-colored colo- nies. Soluble green pigment. On continued cultivation, green color tends to become cream-colored to brownish. Aerial mycelium: Light gray to gray. Sporophores straight, frequently arranged in clumps. Spores elongated, 0.7 by 0.9 to 1.0 micron. Gelatin: Thin, colorless to faintly brown growth, dropping to bottom. No soluble pigment. Slow liquefaction. Agar: Much-folded, cream-colored growth. Aerial mycelium in upper portion of slant white. Soluble, faintly golden pig- ment. Synthetic agar: Slightly folded, cream- colored to brown growth. Aerial mycelium thin, white. Starch agar: Same as on synthetic agar. Glucose agar: Good, brownish growth. Aerial mycelium heavy, cream-colored. No soluble pigment. Broth: Thin, colorless film dropping to bottom. No aerial mycelium. No soluble pigment. Milk: Heavy, cream-colored surface growth. No aerial mycelium. Not coagu- lated; slowly peptonized. Potato: Folded, brown growth. Trace of white aerial mycelium in upper, drier por- tions of growth. No soluble pigment. Sucrose slowly inverted. Starch is actively hydrolyzed. Nitrites weakly produced from nitrates. Antagonistic properties: Positive. Source: Isolated from the soil of potato fields near Berlin. Habitat: Soil. 138. Streptomyces iponioeae (Person and Martin, 1940) Waksman and Henrici, 1948. {Actinomyces ipomoea (sic) Person and Martin, Phytopath., SO, 1940, 313; Waksman and Henrici, in Manual, 6th ed., 1948, 958.) i.po.moe'ae. ML. noun. Ipomoea generic