Page:Bergey's manual of determinative bacteriology.djvu/836

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ORDER V. ACTINOMYCETALES

name of the sweet potato; M.L. gen. noun ipomoeae of Ipoinoea. Conidia on glucose-casein agar: Ellipsoi- dal to elliptical, 0.9 to 1.3 by 1.3 to 1.8 mi- crons. Gelatin: After 25 days at 20° C, scant growth, no aerial mycelium; no soluble pigment; liquefaction. Agar: Moderate growth in the form of small, shiny, crinkled colonies both on the surface and imbedded in the medium, sil- ver-colored. Synthetic agar: Abundant growth, mostly on surface of medium, moderately wrinkled, olive-yellow. Starch agar: Growth moderate, smooth, deep in medium, ivory-colored. Aerial mycelium white with patches of bluish green. No soluble pigment. Complete hy- drolysis after 12 days. Milk: Growth in form of ring; hydroly- sis, without visible coagulation. Potato: Growth moderate, light brown, shiny, wrinkled. No aerial mycelium. No soluble pigment. Starch is hydrolyzed. No growth on cellulose. Nitrites are produced from nitrates. Antagonistic properties: Positive. Source: Isolated from diseased sweet- potato (Ipomoea sp.) tubers and small rootlets from several localities in Louisiana. Habitat: Found in sweet potatoes so far as known. 139. Streptoniyces poolensis (Tauben- haus, 1918) Waksman and Henrici, 1948. {Actinomyces poolensis Taubenhaus, Jour. Agr. Res., 13, 1918, 446; Waksman and Hen- rici, in Manual, 6th ed., 1948, 949.) poo.len'sis. M.L. adj. poolensis pertaining to Poole; named for Prof. R. F. Poole, a plant pathologist. Description taken from Waksman (Soil Sci., 8, 1919, 140). Aerial mycelium: Fine, branching; spirals usually not seen. Conidia ellipsoidal. Gelatin stab: Liquefaction, with small, brownish flakes in fluid. Agar: Yellowish, translucent growth. Synthetic agar: Thin, colorless, spread- ing growth. Aerial mycelium white to gray. Starch agar: Restricted, cream-colored growth. Glucose agar: Growth abundant, light brown, glossy, raised center, entire. Glucose broth: Thin, brownish ring. Litmus milk: Brownish ring; coagulated; peptonized, with strongly alkaline reaction. Potato: Thin, reddish brown; medium becomes purplish. Faint trace of soluble brown pigment. Starch not hydrolyzed. Nitrites produced from nitrates. Aerobic. Optimum temperature, 37° C. Antagonistic properties: Positive. Source: Isolated from a sweet potato "pox." Habitat: Unknown. 140. Streptoniyces tumuli (Millard and Beeley, 1927) Waksman, 1953. {Actinomyces tumuli Millard and Beeley, Ann. Appl. Biol., 14, 1927, 296; Waksman, in Waksman and Lechevalier, Actinomycetes and Their Antibiotics, Baltimore, 1953, 117.) tu'mu.li. L. mas.n. tumulus a mound; L. gen. noun tumuli of a mound. Gelatin: Beaded growth. No aerial my- celium. No soluble pigment. Rapid lique- faction. Agar: Good, lustrous, slimy, gray growth. No aerial mycelium. No soluble pigment. Synthetic agar: Gray growth penetrating into the medium, later becoming darkly opaque. Aerial mycelium arises on center of growth, at first white, later becoming pale gray. Surface of growth covered with colorless drops leaving small, black craters. No soluble pigment. Glucose agar: Wrinkled, pale gray growth. White aerial mycelium arising in concentric rings around a dark bare center. Soluble, olive-colored pigment. Broth: Large, spherical, white colonies. No soluble pigment. Milk: Good growth. No aerial mycelium. Coagulated; slightly peptonized. Potato: Heavy, slimy, black growth. No aerial mycelium. Plug becoming grayish brown. Starch is hydrolyzed.