Page:Bergey's manual of determinative bacteriology.djvu/837

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FAMILY III. STREPTOMYCETACEAE
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Nitrites produced from nitrates. Grows well at 37° C. Source: Isolated from a mound scab of mangels. Habitat: Mangels, so far as known. 141. Streptomyces listeri (Erikson, 1935) Waksman and Henrici, 1948. (Actino- imjces listen Erikson, Med. Res. Council Spec. Rept. Ser. 203, 1935, 36; Waksman and Henrici, in Manual, 6th ed., 1948, 961.) lis'te.ri. M.L. gen. noun listeri of Lister; named for Dr. Joseph Lister, father of anti- septic surgery. Vegetative growth: Long slender fila- ments, many loosely wavy, forming a dense spreading mycelium which rapidly grows into a membrane on most media. Aerial mycelium: Very slow and incon- stant in appearance, short and straight; conidia ellipsoidal. Gelatin: Slight liquefaction; round white surface colonies; after 45 days, confluent skin, almost completely liquefied. Agar: Smooth, round, moist, cream- colored, margin depressed, center ele- vated, closely adherent; becoming umbili- cated, wit^i a myceloid margin. Glucose agar: Cream-colored, glisten- ing, pinpoint colonies; later aggregated in convoluted skin. Glycerol agar: Abundant, moist, cream- colored growth, colonies elevated, piled up; powdery white aerial mycelium. After 20 days, skin deeply buckled; colorless with e.xuded drops. Ca-agar: Poor growth, a slight biscuit- colored membrane. Potato agar: After one week, extensive growth, colorless submerged colonies, warted surface; dirty pink coloration after 2 weeks; scant white aerial mycelium after 4 months. Dorset's egg medium: No growth. Blood agar: Small, round, cream-colored colonies, smooth translucent surface; no hemolysis. Serum agar: Small, irregular, moist, cream-colored colonies, tending to be heaped up; later somewhat transparent. Inspissated serum: Abundant growth, colorless shiny colonies, centrally elevated, becoming confluent. Broth: Small, round, white colonies in sediment. Glucose broth: Small, white, nodular colonies; later abundant flocculi. Synthetic sucrose solution: Delicate white colonies in suspension and in sedi- ment. Litmus milk: Coagulation. No change in reaction. Potato plug: Abundant, dull, brownish, wrinkled skin with white aerial mycelium; large, stellate, fluffy, white colonies in liquid at base. Source: Isolated from human material. Strain from Lister Collection. Habitat: Unknown. 142. Streptomyces galtieri Goret and Joubert, 1951. (Ann. Parasitol. Hum. et Comp., 26, 1951, 118.) gal.ti.e'ri. M.L. gen. noun galtieri of Galtier; named for Prof. Galtier of the Veterinary School, Lyons, France. Vegetative growth: Wavy mycelium branched in a monopodial form, 1 micron in diameter. On agar, two types of colonies are produced: one is small, flat, regular and white; the other large, thick, irregular and yellowish. Aerial mycelium: Producing spirals. Conidia ellipsoidal, 0.8 by 0.8 to 1.5 microns. Gelatin: Poor, flaky, white growth. Limited liquefaction. Agar: Poor, thin, yellowish growth. Aerial mycelium powdery, white. Soluble brown pigment. Synthetic agar: Small colonies. Aerial mycelium powdery, white. No soluble pig- ment. Peptone agar: Limited, cream-colored growth. Aerial mycelium powdery, white. Reddish brown soluble pigment weakly produced. Starch: Thin colonies. Aerial mycelium powdery, white. No soluble pigment. Glycerol potato: Reddish orange puncti- form colonies growing together. A thick crust. Limited white aerial mycelium ap- pearing very slowly. No soluble pigment.