This page has been validated.
INSECTARY: USDA, ARS, SJVASC, Parlier, CA
SOP Rearing Procedure: PTB (Rev. October 2007)
Peach Twig Borer, Anarsia lineatella Zeller; Lepidoptera: Gelechiidae
Origin: 2004; Fresno County; Parlier, CA; U.C. Davis, Kearney Agricultural Center; Laboratory Colony
Diet/Culture: Lima Bean Agar Diet in 1-ounce Cups (See Appendix)
Tasks Performed: Weekly
| Day | Procedure |
| Monday | Harvest/Replace Egg Sheets (→ 50°F); Pull Diet Trays for Tomorrow |
| Tuesday | Infest Diet Cups; Check Sugar-water Vials |
| Wednesday | Harvest Pupae & Set up E/L Containers |
| Thursday | Harvest/Replace Egg Sheets (→ 50°F); Discard Oldest E/L Containers |
| Friday | Move Eggs from 50°F Incubator to Shelf in Rm. #1 (80°F); Check Sugar-water Vials |
Harvest/Replace Egg Sheets (E/S):
- Materials:
- PTB E/S with eggs
- PTB replacement E/S
- Forceps
- ‘S’-weights
- ½ pint jars with solid lids and rings
- Procedure – performed on shelves in holding room:
- Carefully remove ‘S-weight’ from top of egg sheets
- Using forceps, carefully remove the sheets from the lid of the E/L containers
- Distribute egg sheets evenly between six (6) ½-pint jars and close jars with solid cap and ring
- Place jars in 50°F incubator in holding room
- Replace egg sheets on lids of E/L containers – 4 sheets per lid
- Put one ‘S-weight’ on top of each egg sheet
Refill/Replace Sugar-water Vials (10% Fructose):
- Materials:
- 7-dram clear plastic vials with bridal veil material held tightly over top with two rubber bands
- 10% fructose solution
- Petri® dish top or bottom
- Procedure – performed on shelves in holding room and over sink in handling room:
- Fill sugar-water vial with 10% fructose solution
- Invert vial(s) by placing Petri dish on top; hold together; and invert in one smooth motion
- Carefully set one inverted sugar-water vial on top center of each E/L container
- Carefully remove and refill or replace any vials that are less than 1/3 full
- To avoid spills, lift vials straight up, smoothly – but not too slowly
1
