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INSECTARY: USDA, ARS, SJVASC, Parlier, CA
SOP Rearing Procedure: PTB (Rev. October 2007)
Peach Twig Borer, Anarsia lineatella Zeller; Lepidoptera: Gelechiidae
Infest cups – Set up new colony:
- Materials:
- PTB neonate larvae
- PTB cups (1 oz.) with fresh diet
- Small camel hair brush
- Paper lids
- Procedure – performed under fumehood:
- Using a small camel hair brush, infest each diet cup with 3-4 larvae
- Place the larvae on the underside of the lid
- Place lid on cup and push into place, making sure it is fully seated in cup
- Label trays and place on shelf in holding room for development
Set up Egg Layers (E/L):
- Materials:
- PTB pupae
- Oviposition containers with lids with bridal veil centers
- Larval forceps
- Procedure – performed under fumehood:
- Gently harvest PTB pupae from cups
- Place pupae from about 3 trays into each E/L container
- Place E/L containers on shelf in holding room
- Place 1 or 2 egg sheets on top of container with ‘S’-weights
- Place sugar-water vial on top center of lid
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